1. Introduction

Background research

Seeing that many people is starting to use different types of water bottles, there was a need to know which material is best for preventing bacteria growth.

Wanting to know if drinking directly from the bottle affects the bacteria in the water, some research was done on the cultivation of bacteria in saliva, as well as the presence of bacteria in bottled water.

Kat (2005) reported that saliva does infact cultivate bacteria and has a bad reputation for it. Saliva is warm and dark. So when the mouth has food stuck in places, the environment allows bacteria to thrive well. However, most of the bacteria found in saliva are friendly and only causes bad breath at times.

Erik (1999) reported that bacteria found in bottles do not only come from the bottle itself and its material but the water it contains would also have bacteria. Not all bottled water we drink are purer than tap water, some even contain even more harmful bacteria than the strict limit given by government agencies.

Goat (2010) reported that different kinds of bottled water contains different kinds of bacteria. Bacteria commonly found in carbonated or distilled water contains bacteria that are at a non-detectable level. However in the bottled spring water, gram-negative bacteria can be found.

Leung B., Liu S. (n.d) reported that here are many different types of bacteria. Thus, bacteria can be classified by their form, elevation, margin, surface, opacity and chromogenesis also known as pigmentation.

Hypothesis

If the water is kept in the plastic bottle, then bacteria will grow the fastest.

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